Method of processing dried samples using digital microfluidic device

ABSTRACT

Methods are provided for the preparation of a sample using a digital microfluidic platform and the optional subsequent mass analysis of an extracted analyte. A sample is dried, optionally on a solid phase support, and contacted with digital microfluidic array. An analyte present within the dried sample is extracted into an extraction solvent by electrically addressing the digital microfluidic array to transport a droplet of extraction solvent to the dried sample spot. The extracted sample may be dried and subsequently processed on the digital micro fluidic array for derivatization. The digital microfluidic device may further include an integrated microfluidic channel having an output aperture, and the method may further include contacting a droplet containing extracted analyte with the microfluidic channel and applying a suitable electric field for generating nano-electrospray, thereby enabling the device to be directly interacted with a mass analysis device.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. patent application Ser. No. 13/695,869, titled “METHOD OF PROCESSING DRIED SAMPLES USING DIGITAL MICROFLUIDIC DEVICE”, now U.S. Pat. No. 10,232,374, which is a U.S. National Phase Application Under 35 U.S.C. § 371 of International Application No. PCT/CA2011/050205, titled “METHOD OF PROCESSING DRIED SAMPLES USING DIGITAL MICROFLUIDIC DEVICE”, which claims priority to U.S. Provisional Application No. 61/331,679, titled “METHOD OF DIGITAL MICROFLUIDIC SAMPLE PREPARATION FOR MASS ANALYSIS OF METABOLIC DISORDERS” and filed on May 5, 2010, the entire contents of which are incorporated herein by reference.

BACKGROUND

This invention relates to methods of processing of dried samples for subsequent analysis. Blood samples stored as dried blood spots have emerged as a useful sampling and storage vehicle for clinical and pharmaceutical analysis in a wide range of applications. For example, the Newborn Screening Ontario facility at the Children's Hospital of Eastern Ontario evaluates dried blood spot samples from approximately 140,000 babies each year for 28 inherited diseases. In each screening test, a dried blood spot sample is collected and then mailed to facility for analysis by tandem mass spectrometry (MS/MS). Unfortunately, this technique is slowed by an extensive sample preparation regimen (including excision/punching, extraction, evaporation, resolubilization, and derivatization), and in addition, high-throughput screening typically requires robotic sample handling.

The success of dried blood spot sampling and MS/MS for newborn screening has led to a surge in popularity for similar techniques for a wide spectrum of applications in clinical labs and the pharmaceutical industry. Dried blood spot sampling methods allow for the collection of small amounts of sample and are convenient for long-term storage and cataloguing. MS/MS methods allow for the unambiguous identification and quantification of many different analytes in a single shot.

Unfortunately, the throughput and turn-around-time associated with this technique are problematic as a result of time-consuming sample preparation. In particular, the off-line sample preparation of blood spots on filter paper necessitates the labor intensive and time consuming steps of extraction via centrifugation, in which the analyte is obtained in a supernatant. Furthermore, the maintenance of instruments (sample preparation robots and mass spectrometers) and plumbing (capillary tubes and associated connections) requires many hours of laboratory-time, which reduces the throughput of such analyses. In addition, the costs are magnified by the scale of operation (for example, nearly 150,000 samples are processed a year in Ontario alone).

SUMMARY OF THE DISCLOSURE

Methods are provided for the preparation of a sample using a digital microfluidic platform and the optional subsequent mass analysis of an extracted analyte. A sample is dried, optionally on a solid phase support, and contacted with digital microfluidic array. An analyte present within the dried sample is extracted into an extraction solvent by electrically addressing the digital microfluidic array to transport a droplet of extraction solvent to the dried sample spot. The extracted sample may be dried and subsequently processed on the digital microfluidic array for derivatization. The digital microfluidic device may further include an integrated microfluidic channel having an output aperture, and the method may further include contacting a droplet containing extracted analyte with the microfluidic channel and applying a suitable electric field for generating nano-electrospray, thereby enabling the device to be directly interfaced with a mass analysis device.

Accordingly, in one aspect, there is provided a method of sample preparation comprising the steps of: providing a solid phase support comprising a dried sample; providing the solid phase support at a first location between an upper plate and a lower plate of a two-plate digital microfluidic device, wherein the first location is dropwise addressable under actuation of the digital microfluidic device; providing an extraction solvent at an additional location that is dropwise addressable under actuation of the digital microfluidic device; actuating the digital microfluidic device to transport a droplet of the extraction solvent to the first location; and incubating the droplet of the extraction solvent and extracting an analyte in the dried sample.

A further understanding of the functional and advantageous aspects of the invention can be realized by reference to the following detailed description and drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

[Embodiments will now be described, by way of example only, with reference to the drawings, in which:

FIG. 1 illustrates steps in sample processing by digital microfluidics. The top image is a schematic of a digital microfluidic device which allows for the processing of 3 blood samples simultaneously. The bottom image is a sequence of frames from a movie (left-to-right) depicting several stages in sample processing including: (1) a dried blood sample; (2) mixing and incubating an extracted droplet with the sample; (3) a droplet containing sample extractate after translation away from the dried sample; (4) a dried extract; (5) mixing and incubating a derivatization reagent droplet with the dried extract; and (6) the dried, derivatized product.

FIG. 2 illustrates three digital microfluidic methods for processing different sample formats, where (a) shows a droplet of blood spotted directly onto the device surface and allowed to dry, (b) In method 2 shows a punch from filter paper bearing dried blood that is positioned on the device surface.

FIG. 3 illustrates the steps in processing blood samples for quantification of amino acid by tandem mass spectrometry. (a) Reaction scheme involving derivatization of the extracted amino acid, followed by derivatization with n-butanol, followed by the formation of a daughter ion by collision induced dissociation in the mass spectrometer. (b) Mass spectrum generated from primary analysis of derivatized phenyalanine (Phe). (c) Mass spectrum generated from the secondary analysis of derivatized Phe showing the loss of 102 amu as a result of collision induced dissociation.

FIG. 4 provides schematics showing the hybrid digital microfluidic device used for in line mass spectrometry analysis. FIG. 3(a) shows the individual layers forming the device, and FIG. 3(b) shows the integrated device.

FIG. 5 provides calibration curves generated by digital microfluidic sample preparation for quantification of three amino acids in blood.

FIG. 6 provides a comparison of Met, Phe, and Tyr concentrations in normal (green) and spiked (red) blood samples as biomarkers for homocystinuria, phenylketonuria, and tyrosinemia, respectively. The dashed lines indicate the upper levels for normal concentrations in newborn blood samples. Each data point represents at least four replicate measurements, and error bars represent.+−0.1 S.D.

FIGS. 7 (a)-(c) shows a series of frames from a movie (top-to-bottom) demonstrating derivatization and extraction of amino acids, resolubilization in solvent, and analyte solution on a hybrid microfluidic device.

FIG. 8 is an image of sample spraying from the fabricated emitter into a mass spectrometer inlet.

FIG. 9 plots the secondary analysis spectra of (a) Phe and (b) d5-Phe generated from blood samples.

FIG. 10 illustrates the analysis of amino acids in dried blood spots by digital microfluidic methods, where (a) and (b) show frames from a movie depicting sample processing of 3.2 mm diameter punch of a dried blood spot on filter paper by digital microfluidics, and (c) provides a graph of Phe concentrations measured by the digital

FIG. 11 shows a one-sided open DMF device with one carrier pre-loaded with reagents attached to a first substrate.

DETAILED DESCRIPTION

As required, embodiments of the present invention are disclosed herein. However, the disclosed embodiments are merely exemplary, and it should be understood that the invention may be embodied in many various and alternative forms. The Figures are not to scale and some features may be exaggerated or minimized to show details of particular elements while related elements may have been eliminated to prevent obscuring novel aspects. Therefore, specific structural and functional details disclosed herein are not to be interpreted as limiting but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present invention. For purposes of teaching and not limitation, the illustrated embodiments are directed to methods of processing dried samples using a digital microfluidic device.

As used herein, the terms, “comprises” and “comprising” are to be construed as being inclusive and open ended, and not exclusive. Specifically, when used in this specification including claims, the terms, “comprises” and “comprising” and variations thereof mean the specified features, steps or components are included. These terms are not to be interpreted to exclude the presence of other features, steps or components.

As used herein, the terms “about” and “approximately, when used in conjunction with ranges of dimensions of particles, compositions of mixtures or other physical properties or characteristics, is meant to cover slight variations that may exist in the upper and lower limits of the ranges of dimensions so as to not exclude embodiments where on average most of the dimensions are satisfied but where statistically dimensions may exist outside this region. It is not the intention to exclude embodiments such as these from the present invention.

As used herein, the term “exemplary” means “serving as an example, instance, or illustration,” and should not be construed as preferred or advantageous over other configurations disclosed herein.

In a first embodiment, a method of sample preparation is provided in which a digital microfluidic array is employed to extract and prepare an analyte for subsequent analysis. The sample preparation method is especially suitable for use with a subsequent mass analysis step such as a tandem mass spectrometry.

FIG. 1 illustrates a digital microfluidic device 100 for performing a method according to a first embodiment. The device 100 includes an insulating substrate 105 having formed thereon an array 110 of digital microfluidic elements that can be electrically actuated to transport droplets between array elements. The array elements each include a conductive surface that is coated with a hydrophobic insulator. For example, the device may be formed on a glass substrate, onto which patterned chromium electrodes are formed by photolithography and etching, and coated with Parylene-C and Teflon-AF.

Each element is electrically connected to a contact pad (not shown) for electrically addressing the array. The array may further include reagent reservoirs 120 that are suitable for containing a reagent volume. An array element 125 adjacent to a reservoir may be actuated to form reagent droplets and transport the reagent droplets to samples 130 within the array. As shown in FIG. 1, the digital microfluidic array 110 may include multiple regions in which various samples may be processed in parallel. Additionally, side walls and a top plate may also be provided for forming a dual-layer device, in which array elements may be actuated based on a voltage difference between the array and an electrode on the top plate electrode. For example, the top plate may be transparent and may comprise an unpatterned indium tin oxide (ITO) coated glass substrate coated with a hydrophobic material such as Teflon-AF™.

The bottom portion of FIG. 1 provides a series of images from a movie illustrating an example of the present embodiment, in which an amino acid analyte is extracted from a dried blood spot and derivatized for a subsequent mass analysis assay. As shown in the Figure, the images correspond to a single region 140 of the illustrated digital microfluidic device 100.

In image 1 in the bottom portion of FIG. 1, a droplet of blood is provided to the digital microfluidic array and dried to form a dried blood spot 145. It is to be appreciated that the sample may be dispensed directly onto the array, or may be dispensed onto a sample reservoir and the array may be electrically addressed to extract a droplet from the sample reservoir and transport the droplet to an element of the array.

An extraction solvent is then provided to the array, and may be provided by dispensing the extraction solvent to an extraction solvent reservoir 150. As shown in image 2, the array is then electrically addressed to transport one or more droplets 155 of extraction solvent to the dried blood spot. The extraction solvent is incubated (it has been found that approximately 5 to 10 min is sufficient to incubate the extraction solvent) over the dried blood spot and analyte present in the dried blood spot is extracted into the extraction solvent. It will be understood that a suitable incubation time to extract the analyte may depend on the properties of the analyte and extraction solvent. In image 3, the array is again electrically actuated to transport the extraction solvent to a second array element 160, where it is subsequently dried, as shown in image 4.

In image 5, the array is electrically addressed to transport one or more droplets 170 of a derivatization reagent from reagent reservoir 165 to the second array element 160. The derivatization reagent is incubated (for example, for approximately 5 to 10 min) to solubilize and derivatize the analyte previously dried onto second element 160. It will be understood that a suitable incubation time to solubilize and derivatize the analyte may depend on the properties of the analyte and the derivatization solvent. In image 6, the derivatization reagent droplet is evaporated to provide a dried derivatized analyte spot 175.

Although embodiments disclosed herein illustrate various methods using blood as the sample matrix, it is to be understood that the sample need not be blood, and may be or may include any suitable sample matrix, such as, but not limited to, whole blood, serum, plasma, urine, sputum, and cerebral spinal fluid.

For example, as shown in FIG. 2(a), a sample 180 could be spotted directly and dried onto a digital microfluidic device 100 at the point of collection, after which the device could be directly processed or transported to a remote testing location for analysis. If the sample is spotted directly onto the device, it is necessary for the device to be provided at the point of collection. This may be achieved by providing only a component of the device, such as the bottom plate of a two-plate digital microfluidic device. After having received the spotted plate at the laboratory, the full device could be assembled and electrically connected to a device controller. It is to be understood that embodiments involving sample spots dried directly onto the surface of a digital microfluidic array device may be performed using a single plate digital microfluidic device, or a two-plate microfluidic device.

Alternatively, the sample may be spotted onto a solid phase support, such as an exchangeable carrier or exchangeable sheet, as disclosed in International Patent Application No. PCT/EP2009/062657, published as Publication No. WO 2010/037763, titled “EXCHANGEABLE CARRIERS PRE-LOADED WITH REAGENT DEPOTS FOR DIGITAL MICROFLUIDICS”, and filed on Sep. 30, 2009, which is incorporated herein by reference in its entirety. The exchangeable carrier is an electrically insulating sheet having a hydrophobic surface, where the insulating sheet may be contacted with a digital microfluidic array device to form an external surface of the device. After contacting the exchangeable carrier with the digital microfluidic array, the digital microfluidic array may be actuated to transport droplets that are provided on or in contact with the exchangeable carrier. This allows for repeated use of the device without requiring device disposal or decontamination of the device surface.

Although Publication No. WO 2010/037763 disclosed the use of a exchangeable carrier for providing a exchangeable device component including dried reagents, the exchangeable sheets may additionally or alternatively employed to remotely obtaining a dried sample and providing the dried sample to another location (such as a laboratory) for testing. The sample is dried and spotted onto the exchangeable carrier prior to device assembly. The sample is dried at a location such that the dried sample spot is accessible to droplets actuated over the front hydrophobic surface of the exchangeable sheet after attachment to the digital microfluidic device. The dispensing of the sample onto an appropriate location of the exchangeable carrier may be achieved using alignment marks or features, or, for example, using an external masking device that places an aperture at the appropriate location to guide the dispensing process. The exchangeable carrier may also include dried reagents at one or more pre-selected positions, where the positions are selected to be accessible to droplets actuated over the front hydrophobic surface of the exchangeable carrier after attachment to the digital microfluidic device. The exchangeable carrier with a dried spot on a surface thereof may be applied to the surface of a single plate digital microfluidic device, or to the channel-facing surface of one or both of the top and bottom plates of a two-plate digital microfluidic device.

In an alternative embodiment, the sample may be first dried onto a solid phase support, after which at least a portion of the solid phase support is locally contacted with a location on the digital microfluidic for analyte extraction and optional further sample processing steps. This allows for the remote collection of dried sample in a simple and convenient format for subsequent processing with a digital microfluidic device.

An example of this embodiment is shown in FIG. 2(b), where a filter paper punch 185 bearing dried blood is contacted with a surface of a microfluidic device at a location that is dropwise accessible under actuation of the digital microfluidic device. In FIG. 2(b), the filter paper punch is positioned over a digital microfluidic element and contacted with the surface of the first plate as shown. Prior to processing the sample, the top plate of the digital microfluidic device is installed to complete the assembly of the two-plate device (as described above, a gap or channel is formed by a spacer layer provided between the upper and lower plates of the digital microfluidic device). This method allows for the convenient placement of the solid phase support at any internal addressable location of the digital microfluidic array. Alternatively, a two-plate digital microfluidic device may be pre-assembled, and the filter paper punch may be inserted into the gap between the two plates of the digital microfluidic device and positioned at a pre determined location that is dropwise addressable by the digital microfluidic array. Any of the surfaces of the digital microfluidic array may be initially contacted with a exchangeable carrier, and the solid phase support may contact the digital microfluidic device indirectly through contact with the exchangeable carrier.

After having provided the solid phase support at a suitable location between the upper and lower plates of the digital microfluidic device, where the suitable location is in accessible to droplets actuated by the digital microfluidic device, the digital microfluidic array may be actuated to transport one or more extraction solvent droplets to the location of the solid phase support for incubation and extraction of the dried analyte. The extraction and other sample processing steps, such as derivatization of the extracted analyte, may further be performed as described above.

The solid phase support has a thickness that is selected to be compatible with droplet actuation in a digital microfluidic device. For example, a suitable thickness range for a two-plate digital microfluidic device is approximately 90 to 450 microns. As noted above, a suitable solid phase is filter paper, which has a thickness that is compatible with digital microfluidic devices. Other suitable solid phase supports include other porous materials, such as, but not limited to paper, cellulose filters, nitrocellulose, polymer based monoliths such as porous polymer monoliths, and hydrogel forming materials. It has been shown (see below) that when a porous solid phase support such as filter paper is sandwiched between two plates of a digital microfluidic device, the support need not be adhered or otherwise fixed to the device, and is held in place after being contacted with a droplet by inherent forces such as capillary forces. In an alternative embodiment, a non-porous solid phase may be employed, for example, as described above in the context of removable carriers. In another embodiment, a punched non-porous solid phase may be adhered or secured to a surface of the digital microfluidic array using one of many suitable techniques, including bonding methods such as thermal bonding and/or gluing. The solid phase support may be selected to have a hydrophobic surface in order to support the transport of a droplet from a location where the droplet is contacting the solid phase support to a location elsewhere on the digital microfluidic array.

The lateral extent (e.g. surface area and/or diameter) of the solid phase support is sufficiently small to support the actuation of droplets to and from the solid phase support, In some cases, it may be beneficial to limit the lateral extent of the solid phase support to less than one array element. For example, this can allow the transport of droplets along neighbouring channels without contacting the solid phase support. However, it is to be understood that the lateral extent of the solid phase support need not be smaller than an array element, and the lateral extent of the solid phase support may overlap with neighbouring array elements, as demonstrated in FIG. 10.

For example, a larger lateral extent of the solid phase support will provide a larger radial extent of dried solvent, and a correspondingly higher amount of analyte for extraction. In some applications, it may be desirable to provide a greater number of analyte molecules, particularly in applications where the analyte concentration range of interest is near to the limit of detection of an analytical method employed for subsequent detection.

It may also be useful to provide, on a single digital microfluidic array, multiple solid phase supports, where each solid phase support contains a common dried sample. The multiple solid phase supports may be subjected to extraction steps as described above, where the extraction steps are performed in parallel or in serial format. The extracted analyte from the multiple solid phases may be dried onto a common array element in order to concentrate the extracted analyte.

In another embodiment, multiple solid phase supports may be provided on a single digital microfluidic array, where the solid phase supports contain analyte or analytes from different samples for parallel processing on a single digital microfluidic array. This allows for multiplexed extraction from multiple samples, and is beneficial in further reducing labor costs and turn around time. For example, such an embodiment may be useful for the multiplexed extraction and processing of analyte in applications involving high-throughput screening.

After utilizing the digital microfluidic array for the extraction and derivatization of the analyte as described above (either using direct sample deposition and drying or indirect dried sample processing), an assay for the analyte may be subsequently performed using a mass analyzer. To prepare the derivatized analyte for mass analysis, the derivatized analyte is resuspended in a solvent compatible with the subsequent mass analysis step.

The extraction solvent may additionally contain an internal standard for use with the subsequent mass analysis step. The standard may include a concentration of isotopically labeled analyte. The mass analysis may involve analysis by collision-induced dissociation for tandem mass analysis. In another embodiment, the resuspended analyte may be first provided to a chromatographic separation system (such as a high performance liquid chromatography system) prior to subsequent analysis of the separation system eluent with a mass analysis device.

In one embodiment, the analyte is one or more amino acids, fatty acids (acylcarnitines), organic acids, and a combination thereof. The extraction solvent in this case is preferably methanol. A suitable derivatization step is illustrated in FIG. 3(a) for the non-limiting case of amino acid (amino acid) analyte, in which a derivatization reagent comprising HCl-butanol transforms each amino acid to its corresponding butyl ester (derivatized amino acid), and subsequent formation of daughter ions via collision induced dissociation makes each analyte convenient to quantify by multiple reaction monitoring. The capacity to carry out similar processes in parallel for many different amino acids simultaneously with minimal steps makes the present digital microfluidic and MS/MS method a useful tool for a wide range of applications, including the screening of metabolic disorders on a large scale.

FIGS. 3(b) and 3(c) provide illustrative primary (MS1) and secondary (MS2) mass spectra for the amino acid, phenylalanine, with peaks at m/z 222 and 120. This technique, using collision induced dissociation and MS/MS, is useful for selectively analysing such species because they exhibit a characteristic loss of butylformate (HCOOC₄H₉, 102 D), making them easy to identify.

In one embodiment, more than one extraction step may be performed using the digital microfluidic array for the purpose of extracting multiple analytes that are beneficially processed using different extraction and/or derivatization solvents. In a one embodiment, the multiple extraction methods are performed on a common digital microfluidic platform. The multiple extraction methods may be performed serially using a common dried sample spot or in parallel using two separate dried sample spots, where the dried sample spots may be dried directly onto the digital microfluidic device, or dried onto an intermediate matrix as described above. The final droplets containing the extracted analyte, in which the droplets are provided in a fluid that is compatible with a subsequent mass analysis step, may be combined and provided to the mass analyzer in a single aliquot for multiplexed mass analysis. In a non-limiting example, the marker succinulacetone may be extracted in order to perform an assay for tyrosinemia type I. The extraction method is similar to that discussed above, in which an acidic extraction solvent containing hydrazine is provided to the digital microfluidic array. Preferably, this extraction solvent comprises an acetonitrile/water/formic acid solution having relative concentrations of 80:20:0.1% by volume, respectively, and further containing 0.1% hydrazine monohydrate and isotopically labeled succinylacetone (¹³C⁵-succinylacetone), as discussed in Turgeon et al. (C. Turegeon et al., Clin. Chern. 54, 657, 2008). The array is then electrically actuated to transfer a droplet of the extraction solvent to a dried sample (e.g. a dried blood spot), which may have already been processed according to the above protocol using a different solvent (e.g. methanol). The extraction solvent droplet containing the extract succinulacetone is then transported to a different element of the digital microfluidic array under electrical actuation of the array, where it may be dried and resolubilized in another buffer prior to analysis.

In one embodiment, the digital microfluidic device is interfaced a the mass spectrometer to support a method that may be performed without the need for intermediate manual or robotic liquid handling steps. This overcomes many of the difficulties associated with convention nanoflow electrospray ionization (nESI), which is known to be a complex technique requiring operator expertise and vigilance to achieve reproducible results. For example, this limitation is part of the reason why newborn blood samples are often mailed to a single remote screening facility for processing.

Accordingly, in one embodiment, a nESI device is integrated into the microfluidic device to provide a hybrid digital microfluidic and nESI device that may be formed by standard batch processing. Sample processing is performed as described above, where an analyte is extracted and processed from a dried sample (directly dried onto the digital microfluidic array, or indirectly dried onto a suitable matrix which is contacted with the array), and mass analysis is realized by positioning the hybrid device in front of the mass spectrometer inlet and applying an electrical potential to achieve nESI. This process requires only a few minutes to accomplish and can be implemented by non-experts.

Such an integrated system is illustrated in FIG. 4, which shows the various layers that form the device in FIG. 4(a) and the overall integrated device in FIG. 4(b). As shown in FIG. 4(a), the device comprises a top plate 200, a digital microfluidic layer 205, and a microfluidic channel layer 210. Top plate 205 comprises a non-conductive and substrate, having formed on its lower surface an electrode that is further coated with a hydrophobic material. Preferably, top plate is transparent and comprises an unpatterned indium tin oxide (ITO) coated glass substrate coated with a hydrophobic material such as Teflon-Ar™.

Digital microfluidic layer 205 is similar to the digital microfluidic device described above, and comprises an insulating substrate having formed thereon an array 215 of digital microfluidic elements that can be electrically actuated to transport droplets between array elements. Preferably, the array elements each comprise a conductive surface that is coated with a hydrophobic insulator. For example, the device may be formed on a glass substrate, onto which patterned chromium electrodes are formed by photolithography and etching, and coated with Parylene-C and Teflon-AF. As described above, each element is electrically connected to a contact pad (not shown) for electrically addressing the array. The array preferably further comprises reagent reservoirs 220 that are suitable for containing a reagent volume. Vertical spacing elements 225 are provided between top plate 200 and digital microfluidic layer 205 for forming a planar channel within which fluidic droplets may be transported by electrically addressing the digital microfluidic array.

Digital microfluidic layer 205 further includes a vertical hole 230 located adjacent to array element 235, enabling droplets residing on the digital microfluidic array to be transported to, and contacted with, the aperture of vertical hole 230, whereby hole 230 may be filled under capillary action.

Microfluidic channel layer 210 comprises a microfluidic channel 240 formed in an upper surface of a substrate, and extending to the edge of the substrate. By positioning microfluidic channel layer 210 in contact with the underside of digital microfluidic layer 205 so that an end portion of microchannel 240 is contacted with vertical hole 230, an inlet is formed in microchannel 240 that may be filled by fluid captured by vertical hole 230 under further capillary forces. Accordingly, microfluidic channel 240 may be filled with liquid from a droplet by electrically addressing the digital microfluidic array to transport the droplet to contact vertical hole 230, which leads to the subsequent filling of channel 240 under via capillary forces. The contacting of microfluidic channel layer 210 with digital microfluidic layer 205 also forms an external outlet 245 of microfluidic channel 240. In a preferred embodiment, microfluidic channel outlet 245 is located in the corner of the device. FIG. 4(b) illustrates an integrated device 250 containing a sample droplet 260 and a reagent 270 loaded onto a reagent reservoir.

In another embodiment, the digital microfluidic array is employed to perform a sample preparation method as disclosed above, and the derivatized analyte is solubilized in a fluidic droplet that is compatible with a subsequent mass analysis step. The droplet is transported to contact and fill the microfluidic channel, and the device is positioned in close proximity to the inlet of a mass analysis device. An electrical conductor is then made to contact the liquid in the microchannel, for example, by removing the top plate and placing a wire into the vertical hole 230. Alternatively, a conductor may be made to contact the contents of the microfluidic channel by forming a secondary access hole and inserting the electrical conductor into the secondary hole in such a way as to provide a suitable fluidic seal, thereby preventing leakage from the microchannel. Electro spray may be subsequently generated in a cone emerging from outlet 245 by applying a voltage between the conductor and the inlet of the mass analysis device. In yet another embodiment, the electrical contact point may be made at the top plate (ITO slide) with a suitable contact means such as a soldered wire or an alligator clip. This contact is connected to the MS power supply for applying a voltage between the microfluidic channel outlet and the inlet to the mass spectrometer.

While the present embodiment illustrates the interfacing of a digital microfluidic array with a microfluidic channel located beneath the digital microfluidic array, it is to be understood that the digital microfluidic array may be interfaced with the microfluidic channel in a variety of different geometries. For example, the digital microfluidic array may be interfaced with a microfluidic channel having an inlet that is laterally adjacent to an element of the digital microfluidic array, as disclosed in PCT Application No. PCT/CA2009/001439, published as Publication No. WO/2010/040227, filed Oct. 13, 2009 and titled “Hybrid Digital and Channel Microfluidic Devices and Methods of Use Thereof” which is incorporated herein by reference in its entirety.

The methods provided herein are generally automated and are thus typically faster and less prone to operator error when compared to the conventional techniques in terms of sample preparation and reagent use. Specifically, the methods disclosed herein avoid the need to manually process samples dried on filter paper, namely centrifuging the filter paper in an extraction solvent, which involves laborious and time consuming steps.

The aforementioned embodiments may be applied for a wide range of sample types, analytes, and processing applications. Although embodiments disclosed above have focused on three specific metabolic diseases (homocystinuria, phenylketonuria, and tyrosinemia), it is to be understood that the scope of the various embodiments includes, but is not limited to a wide range of analytes that are compatible with digital microfluidic array based extraction and processing.

Example assays include, but are not limited to, amino acid assays, fatty acid disorders (acylcarnitines), organic acid disorders, and markers for metabolic disorders. Tables 1, 2 and 3 below provide a non-limiting list of various known amino acid disorders, fatty acid disorders, and organic acid disorders, respectively, and their markers. The analytes below merely provide an illustrative list and are not intended to limit the scope of the embodiments provided in the present disclosure.

TABLE 1 List of Amino Acid Metabolic Disorders and their Markers Amino Acid Disorders Marker(s) Argininemia or Arginase deficiency Arginine Citrullinemia-1 or Argininosuccinate Citrulline synthase Argininosuccinic aciduria or Citrulline, Argininosuccinate lyase deficiency argininosuccinic acid Ornithine transcarbamylase deficiency Alanine, Citrulline Carbamoylphosphate synthetase deficiency Alanine, Citrulline Citrullinemia-II or Citrin-mitochondrial Citrulline aspartate-glutamate transporterdeficiency Hyperammonemia-Hyperornithine mia- Ornithine Homocitrullinuria Syndrome Phenylketonuria or Phenylalanine Phenylalanine hydroxylase deficiency Maple Syrup Urine Disease or Branched Leucine, Valine chain ketoacid dehydrogenase deficiency Homocystinuria or Cystathioninebeta- Methionine synthase deficiency Non ketotic hyperglycinemia Glycine Tyrosinemia I or Fumarylacetoacetase Tyrosine deficiency Tyrosinemia II or Tyrosine Tyrosine aminotransferase deficiency Tyrosinemia III or 4-hydroxyphenylpyruvic Tyrosine acid dioxygenase deficiency 5-Oxoprolinuria or Glutathione synthetase 5-oxoproline deficiency Biopterin defects Phenylalanine

TABLE 2 List of Fatty Acid Metabolic Disorders and their Markers Fatty Acid Oxidation Disorders Marker(s) Very long-chain acyl-CoA Tetradecenenoylcarnitine, dehydrogenase deficiency Tetradecanoylcarnitine Long-chain hydroxyacyl-CoA Hydroxyhexadecanoylcarnitine, dehydrogenase deficiency Octadecenenoylcarnitine, Hydroxyoctadecenenoylcarnitine Medium-chain acyl-CoA Octanoylcarnitine, dehydrogenase deficiency Hexanoylcarnitine, Decanoylcarnitine Short-chain acyl-CoA Butyrylcarnitine & dehydrogenase deficiency Isobutyrylcarnitine Tri-functional protein deficiency Hydroxyhexadecanoylcarnitine, Hydroxyoctadecenenoylcarnitine, Glutaric aciduria-II or Multiple acyl- Butyrylcarnitine & CoA dehydrogenase deficiency Isobutyrylcarnitine, Isovalerylcarnitine & 2-methylbutyrylcarnitine, Glutarylcarnitine, Octanoylcarnitine, Tetradecanoylcarnitine Carnitine palmitoyl transferase Free carnitine deficiency-I Carnitine palmitoyl transferase Octadecenenoylcarnitine, deficiency-II Hexadecanoylcarnitine Carnitine/acylcarnitine translocase Octadecenenoylcarnitine, deficiency Hexadecanoylcarnitine Carnitine uptake defect or 2,4- Free carnitine, Dienoyl-CoA reductase deficiency Decadienoylcarnitine Hydroxyacyl-CoA dehydrogenase Hydroxybutyrylcarnitine deficiency or Short/Medium-chain hydroxyacyl-CoA dehydrogenase deficiency Propionic acidemia or Propionyl- Propionylcarnitine CoA carboxylase deficiency

TABLE 3 List of Organic Acid Metabolic Disorders and their Markers Organic Acid Disorders Marker(s) Methylmalonic aciduria or Propionylcarnitine Methylmalonyl-CoA mutase deficiency Cobalamin defects (A, B) Propionylcarnitine Cobalamin defects (C, D) Propionylcarnitine, Methionine Multiple carboxylase deficiency Propionylcarnitine, Hydroxyisovalerylcarnitine 3-Hydroxyisobutyric aciduria Hydroxybutyrylcarnitine Isovaleric acidemia or Isovaleryl- Isovalerylcarnitine & 2- CoA dehydrogenase deficiency methylbutyrylcarnitine 2-Methylbutyrylglycinuria or 2- Isovalerylcarnitine & 2- Methylbutryl-CoA dehydrogenase methylbutyrylcarnitine deficiency 3-Methylcrotonyl-CoA carboxylase Hydroxyisovalerylcarnitine deficiency 3-Hydroxy-3-methylglutaryl-CoA Hydroxyisovalerylcarnitine, lyase deficiency Mitochondrial acetoacetyl-CoA Tiglylcarnitine plus 3- thiolase deficiency or Beta- methylcrotonylcarnitine ketothiolase deficiency Methylglutaconic aciduria or 3- Tiglylcarnitine plus 3- Methylglutaconyl-CoA hydratase methylcrotonylcarnitine deficiency 2-Methyl-3-hydroxybutyryl-CoA Hydroxyisovalerylcarnitine dehydrogenase deficiency Malonic aciduria or Malonyl-CoA Malonylcarnitine decarboxylase deficiency Glutaric aciduria-I or Glutaryl-CoA Glutarylcarnitine dehydrogenase deficiency

It is to be understood that while the preceding embodiments have included a derivatization step for processing the extracted analyte using the digital microfluidic array, this step may not be required for extraction protocols that are suitable for other analytes. As such, the derivatization step, or any further on-chip processing steps, may be optionally performed, as appropriate for a given analyte or application.

Example applications include, but are not limited to, neonatal screening of metabolic disorders (i.e. amino acids and organic acids), other disorders (for example, congenital adrenal hyperplasia, congenital hypothyroidism, biotinidase deficiency and galactosemia), and genetic disorders (e.g. cystic fibrosis and sickle cell diseases).

For example, in each newborn blood spot analysis, a sample is obtained by pricking the subject's heel (or by venipuncture) and allowing a spot of blood to dry on filter paper. The dried blood spot is typically couriered to a lab, where 3.2 mm diameter circular discs are punched, and the analytes are extracted, mixed with isotope-labeled internal standards, derivatized, and then reconstituted for analysis by tandem mass spectrometry (MS/MS). As shown in FIG. 3(a), the derivatization step transforms each amino acid to its corresponding butyl ester (derivatized amino acid) that allows for a characteristic fragmentation pattern (neutral loss of 102) via collision induced dissociation. FIGS. 3(b-c) contains representative primary (MS1) and secondary (MS2) mass spectra for the amino acid, phenylalanine, with peaks at m/z 222 and 120. In addition to amino acids, the same derivatization step butylates acylcarnitines (AC), which serve as markers of inborn errors of fatty acid and organic acid metabolism.

An additional and clinically relevant advantage of the present digital microfluidic methods is the reduction in sample size that may be achieved relative to conventional processing methods. In some cases, the reduction in volume may be approximately 15-20×. This reduction has the potential to be beneficial for applications in which it is desirable to employ small sample volumes, such as in the testing of newborn patients, from which five spots of blood are typically collected for analysis, and in high throughput screening applications.

In the examples provided herein, the small volume required (5×5 μL=25 μL) for certain clinical in-vitro diagnostic assays can be collected as capillary blood with a single needle-prick, but in conventional sample processing methods, the volume (5×75-100 μL=375-500 μL) often requires multiple pricks and tissue-squeezing, which can contaminate the sample with interstitial fluids, invalidating it for testing. Other sample size-related advantages of the present embodiments are a reduction in reagent use (for example, 20 μL vs. 170-450 μL), and a reduction in analysis time (˜1 h vs. >3.5 h). This reduction in reagents and analysis time, combined with the potential elimination of sample preparation robotic liquid handling systems, makes the present methods an attractive option for diagnostic testing in a time of increasing costs for health care.

The following examples are presented to enable those skilled in the art to understand and to practice the present invention. They should not be considered as a limitation on the scope of the invention, but merely as being illustrative and representative thereof.

Example 1 Device Fabrication and On-Chip Processing of Amino Acids

Digital microfluidic devices were fabricated in the University of Toronto Emerging Communications Technology Institute (ECTI) cleanroom facility, using a transparent photomask printed at Norwood Graphics (Toronto, ON). Glass devices bearing patterned chromium electrodes were formed by photolithography and etching and were coated with 2.5 μm of Parylene-C and 50 nm of Teflon-AF. Parylene-C was applied using a vapor deposition instrument (Specialty Coating Systems), and Teflon-AF was spin-coated (1% wt/wt in Fluorinert FC-40, 2000 rpm, 60 s) followed by post-baking on a hot-plate (160° C., 10 min). The polymer coatings were removed from contact pads by gentle scraping with a scalpel to facilitate electrical contact for droplet actuation.

A prototype similar to the device shown in FIG. 1 was fabricated to analyze 5 μL blood samples. As shown in the top image, an array of 88 driving electrodes connects a series of 10 reservoirs dedicated to microliter volumes of sample and reagents. As depicted in the bottom image, blood samples are spotted onto the device and dried, after which the sample is extracted into methanol and the solvent is allowed to evaporate. The extract is then derivatized, and the product is isolated by allowing the solvent to evaporate. The entire process requires 50 min to complete (compared with >3.5 h for clinical laboratories, not including mailing time).

The device featured an array of eighty-eight actuation electrodes (2.2×2.2 mm ea.) connected to ten reservoir electrodes (5×5 mm ea.), with inter-electrode gaps of 40 μm. Devices were assembled with an unpatterned ITO-glass top plate and a patterned bottom plate separated by a spacer formed from four pieces of double-sided tape (total spacer thickness 360 μm). Unit droplets (covering a single driving electrode) were—1.8 μL. To actuate droplets, driving potentials (70-100 V_(RMS)) were generated by amplifying the output of a function generator (Agilent Technologies, Santa Clara, Calif.) operating at 18 kHz. As described elsewhere, droplets were sandwiched between the two plates and actuated by applying driving potentials between the top electrode (ground) and sequential electrodes on the bottom plate via the exposed contact pads. Droplet actuation was monitored and recorded by a CCD camera mounted on a lens.

Blood samples were collected from a healthy adult male volunteer after a 10 h fasting period and were kept at −20° C. until analysis. Immediately prior to use, samples were thawed and evaluated as described.

Working solutions of all amino acids (amino acids) (25, 50, 100 and 500 μm ea.) were prepared in DI water. For derivatization of extracted amino acids, a 3 N HCl-butanol solution was prepared from a mixture of 12 N HCl/neat butanol (1:3 v/v). For analysis of amino acids in blood samples, the extracting solvent (MeOH) contained 25 11M of the appropriate deuterated amino acid (d3-Met, ds-Phe or c4-Tyr). For quantitative analysis of amino acid recovery from blood and for experiments mimicking diseased/healthy infant blood, samples were spiked with 200 11M of the appropriate amino acid (Met, Phe or Tyr). In all experiments, organic solvents were HPLC grade and deionized (DI) water had a resistivity of 18 MO·cm at 25° C.

5-μL droplets containing the sample (i.e., amino acid standards, whole blood or spiked whole blood) were pipetted onto the bottom plate of a device and dried. The top plate was then affixed and two solvents were loaded into the appropriate reservoirs, including MeOH containing 25 μm of deuterated amino acid (extraction solvent), and 3 N HCl-butanol (derivatization solvent). A reservoir volume (10 μL) of extraction solvent was dispensed and driven by digital microfluidic to the dried sample and allowed to incubate (5 min). The extraction solvent was then actuated away from the sample and dried (˜15 min, room temperature) at a second site, after which a reservoir volume (10 μL) of derivatization solvent was dispensed to the dried extract and incubated for 15 min at 75.degree. C. Following the reaction, the top plate was removed and the solvent was allowed to evaporate (˜15 min, room temperature).

Calibration curves were generated by digital microfluidic sample preparation for quantification of (a) methionine (Met), (b) phenylalanine (Phe), and (c) tyrosine (Tyr) in blood. As shown in FIG. 5, data was generated by plotting the intensity ratios of the daughter ions of each amino acid relative to their deuterated internal standard (i.e., d3-Met, d5-Phe, d4-Tyr, respectively) as a function of amino acid concentration. Each data point represents at least four replicate measurements, and error bars represent ±1 S.D. Regression lines were linear with R²>0.996 for each analyte.

For comparison, amino acids were also extracted and derivatized on the macroscale using known methods. Amino acid samples (20 μL) were pipetted and dried in a microcentrifuge tube and extracted in methanol (500 μL, 30 min) containing isotope-labeled internal standards. The solution was centrifuged (13,000 rpm, 15 min), and the supernatant transferred to a second tube and evaporated to dryness using nitrogen. The extractate was then resuspended in 3 N HCl butanol solution (250 μL) to derivatize the amino acids at 65° C. for 20 min, followed by evaporation of the derivatized mixture.

Most samples were processed by digital microfluidic and then were analyzed offline by nanoelectrospray tandem mass spectrometry (nESI-MS/MS). Such samples (stored dry on device or in centrifuge tube until analysis) were reconstituted in 70 μL of acetonitrile/water (4:1 v/v); samples originating from blood were, in addition, passed through PVDF membrane centrifuge filters with 0.1 μm pore diameter (Millipore, ON). Samples were injected into an LTQ Mass Spectrometer (Thermo Scientific) via a fused silica capillary transfer line (100 μm i.d.) mated to a New Objective Inc. (Woburn, Mass.) nanoelectrospray emitter (100 μm i.d. tapering to 50 μm i.d.) at a flow rate of 0.8 μL min, with an applied voltage of 1.7-1.9 kV and capillary temperature of 200° C. Tandem MS/MS analysis was carried out by introducing 30% collision energy to the parent ions and then the fragments over the m/z range of 100-300 were scanned. For amino acid samples, the daughter ions detected in the second mass selection, which exhibit a loss of butylformate (HCOOC₄H₉, 102 m/z), were observed and used for quantification. Spectra were collected as an average of 50 acquisitions, and replicate spectra were obtained for digital microfluidic-derivatized samples of both control and blood.

Some samples were analyzed by nESI-MS/MS in-line on hybrid digital microfluidic microchannel devices bearing an integrated nESI emitter. In these experiments, hybrid devices were mounted on a 3-axis micromanipulator (Edmund Optics, NJ) positioned near the inlet of the LTQ MS. After sample processing, a spray was generated by applying 2.5-3.0 kV to a platinum wire inserted in the access hole (see FIG. 6).

Tandem mass spectrometry was used to (i) quantify amino acids in blood samples and (ii) evaluate the recovery efficiency of the digital microfluidic method. For the former (quantification of amino acid in blood samples), calibration plots were generated by plotting the intensity ratio of daughter ions from the extracted amino acids relative to the those of the internal standards (i.e., Met m/z 104:107, Phe m/z 120:125, and Tyr m/z 136:140) as a function of amino acid concentration in standard solutions (25-500 μM in DI water). Data points included in the calibration plots represent an average of at least 4 replicate measurements, and the data in each plot were fit with a linear regression. Blood samples were then evaluated (with on-chip derivatization and extraction, and measurement by MS/MS relative to internal standards, as above), and the values were compared to the calibration plots to determine the amino acid concentrations. For the latter (evaluation of % recovery), blood samples of known amino acid concentrations were spiked with 200 μM of amino acid standards and extracted (as above). Knowing the total concentration of amino acids in blood spots (e.g. native methionine concentration plus spiked methionine), % recovery was obtained by comparing the concentration values (obtained from calibration curves) vs. the known values.

The % recovery of amino acids was evaluated quantitatively using a fluorescence-based assay. Control samples (Met, Phe or Tyr; 50 μM of each) were processed by digital microfluidic (as above), excluding the derivatization step. The dried extracts were diluted into 95 μL aliquots of borate buffer (20 mM, pH 8.5) in wells in a 96-well microplate. Upon addition of 5 μL of fluorescamine (5 mg/mL in acetone) the microplate was inserted into a fluorescence microplate reader (Pherastar, BMG Labtech, Durham, N.C.) equipped with a module for 390 nm excitation and 510 nm emission. The plate was shaken (5 s) and the fluorescence was measured. As a control, identical samples that had not been extracted were evaluated using the same fluorescent assay. To ensure that controls were processed in identical manner relative to extracted samples, each control sample was spotted on a device, dried and then reconstituted in buffer for analyses. Four replicate measurements were made for each sample and control.

Nanoelectrospray ionization tandem mass spectrometry (nESI-MS/MS) is used to quantify amino acids in samples of blood processed by the digital microfluidic method. Calibration curves with R² greater than 0.996 (FIG. 4) were generated by analyzing standards processed by digital microfluidic at known concentrations from the abundance ratio of each amino acid to its deuterated standard peak in the secondary (MS2) spectra. The calibration curves facilitated measurement of amino acid concentrations in blood samples from a healthy male volunteer. As listed in Table 4, the values obtained were in the expected physiological range and the precision in the method was high with coefficients of variation (CVs) ranging from 5 to 11%.

TABLE 4 Measured (±1 S.D.) and normal adult concentrations of amino acids in blood. Measured Blood Concentration (μM) in an Normal Blood Amino Acid Adult Male Volunteer Concentration (μM) Methionine 25 ± 2 16-33 Phenylalanine 38 ± 2 41-68 Tyrosine 46 ± 5 45-74

Fluorescence and MS/MS were used as an orthogonal test to evaluate the extraction efficiency of the new digital microfluidic technique. The former method relies on fluorescamine, a fluorogenic reagent that exhibits no fluorescence until it reacts with primary amines. Recovery was determined by comparing the fluorescence intensity of multiple samples before and after extraction. In the latter (MS/MS) method, blood samples were spiked with amino acid and recovery was determined by comparing the amino acid concentration (native amino acid plus spiked amino acid) vs. known concentration. As listed in Table 5, the two orthogonal methods (fluorescence and MS/MS) agree and reveal the new technique to be very efficient and recovery was 80% for each standard and blood sample evaluated. As above, the precision of these measurements was high, with CVs ranging from 1 to 10%.

TABLE 5 % Recovery of the DMF method measured by fluorescence and MS/MS (±1 S.D.) % Recovery by % Recovery by Amino Acid Fluorescence in Standards MS/MS in Blood Methionine 98 ± 10 100 ± 1  Phenylalanine 86 ± 9  85 ± 5 Tyrosine 82 ± 10 84 ± 7

To validate the new digital microfluidic method as a platform for analyzing amino acid disorders in blood, spiked blood samples (mimicking diseased states) and non-spiked blood samples (mimicking healthy state) were analyzed by mass spectrometry. FIG. 6 shows a comparison of measured concentration of amino acids in normal and spiked blood samples. The dashed line indicates the threshold value for diagnosis of homocysteinuria (67 μM Met), phenylketonuria (120 μM Phe), and tyrosinemia (150 μM Tyr). As shown, the method is useful for distinguishing between these states.

As shown in FIG. 4, a hybrid digital microfluidic system was fabricated (using process similar to those disclosed above) in which an nESI device was directly integrated with the microfluidic device. The hybrid digital microfluidic-microchannel devices were fabricated in four steps.

First, a DMF bottom substrate (layer 205 in FIG. 4(a)) bearing an array of DMF driving electrodes) was fabricated as described above, but without a Teflon-AF™ coating). The design was similar to that of the DMF-only devices described above, but with fewer electrodes—2 rows of 9 actuation electrodes 215 (2.2x×2.2 mm) and 3 reservoir electrodes 220 (5x×5 mm). Moreover, the substrates were first modified by drilling an access hole 230 (˜2 mm diameter) through the substrate using a micro drill-press before the photolithographic processes, where the hole was drilled adjacent to the planned location of an electrode 235. After patterning the electrodes, the opposite side was first coated with 7 μm of Parylene-C for bonding with substrate 210.

Secondly, a glass substrate 210 bearing a microchannel 245 nanoelectrospray tip 240 formed in Parylene was fabricated. 37 grams of Parylene-C were deposited on piranha cleaned, silanized glass slide (25×55 mm) via vapor deposition. After Cr deposition, a microchannel (25 μm wide×5 mm long) was photolithographically patterned on the substrate by UV radiation (365 nm, 35 mW/cm2, 50 s) using a Karl-Suss MA6 mask aligner (Garching, Germany).

Third, the channel side of substrate 210 was mated to the non-electrode side of substrate 205, placed under pressure in a precision vise (˜20 MPa), and thermally bonded in a vacuum oven (200° C., 24 h). After cooling, the top of substrate 205 was first coated with 2 μm of parylene followed by spin-coating 50 nm of Teflon-AF with a small piece of dicing tape covering the drilled hole. The tape was removed before post-baking on a hot plate (160° C., 10 min).

Fourth, the top plate 200 was assembled with spacers formed from four pieces of double-sided tape as described above for droplet actuation.

Using this hybrid device, droplets are manipulated on the top surface, and are subsequently transferred to microchannels on the bottom of the device through the hole. The principle of operating the hybrid device for on-chip sample analysis is shown in FIG. 7, which shows several frames from a movie demonstrating derivatization and extraction of amino acids, resolubilization in solvent, and analyte solution on a hybrid microfluidic device. A blood sample 310 was first spotted on the device 300 and the amino acids were extracted and derivatized as described above. The dried, derivatized sample 320 was then resuspended in acetonitrile and the droplet 330 was actuated towards the access hole 340 such that it filled the underlying channel 350. The filled channel can be seen at 360 in FIG. 7(c).

As shown in FIG. 8, the fluid in the channel was delivered to a corner 400 of the device and nanoelectrospray 410 was generated by applying a high voltage to the counter electrode 420. Representative mass spectra generated from samples processed and analyzed on-chip are shown in FIG. 9. The entire process requires ˜1 h from sampling to analysis, and requires only the hybrid digital microfluidic device and a mass spectrometer (i.e., no complex nanoflow pumps, robots, samplers, or control software).

Example 2 Direct Digital Microfluidic Processing of Sample Dried on Solid Phase

In the present example, a digital microfluidic method is demonstrated using the direct processing, on a digital microfluidic array, of samples dried onto filter paper. A digital microfluidic array device was fabricated as described in Example 1, except that in the present case, the two plates were separated by six pieces of double-sided tape (total spacer thickness 540 11 m). This increased spacing was provided to accommodate the thickness of the filter paper. A blood sample was collected and dried onto filter paper, and a 3.2 mm diameter filter paper disc was punched for subsequent analysis. The punch was placed onto an element of a digital microfluidic array.

A portion of an experiment is depicted in FIG. 10(a). As shown, a droplet of extraction solvent 510 was dispensed on the digital microfluidic array 500 and driven to the filter paper punch 520, and the extract was then moved away and dried 530 for further processing (i.e., derivatization and solvent exchange, similar to FIG. 1). As can be seen in FIG. 10(b), after wetting, the filter paper punch 520 remains adhered to the surface through capillary forces. This process requires ˜50 min to complete.

To evaluate this digital microfluidic method relative to gold standard practices, a series of punches from blood samples containing various concentrations of Phe were processed by this digital microfluidic method, and punches from the same samples were evaluated using the conventional newborn screening technique. As listed in Table 6 below, a paired t-test revealed no significant difference between the two data sets at a 95% confidence level. To validate the new technique for application to clinical samples, dried blood spot punches from three newborn patients of NSO were evaluated by the digital microfluidic method. As shown in FIG. 10(b), the new technique correctly identified patients 1 and 3 as suffering from phenylketonuria, and patient 2 as being unaffected.

TABLE 6 Measured phenylalanine (Phe) concentration in 3.2 mm dia. punches from filter paper bearing dried blood using digital microfluidic method 2 (left) and standard 15 techniques at NSO (right). A paired t-test (P = 0.05, t = 0.69) revealed no significant difference between the two data sets. Measured Phe Measured Phe Concentration (μM) Concentration (μM) Sample Using DMF Method Using NSO Technique 1 70 70 2 550 548 3 93 88 4 93 92 5 368 302 6 534 539 7 735 871

The foregoing description of the embodiments of the invention has been presented to illustrate the principles of the invention and not to limit the invention to the particular embodiment illustrated. It is intended that the scope of the invention be defined by all of the embodiments encompassed within the following claims and their equivalents.

FIG. 11 shows a one-sided open DMF device with a carrier 10′ that is pre-loaded with reagent depots 12′ for use with a digital microfluidic device 14′ and that is attached to a first substrate 24′. The digital microfluidic device includes an array 16′ of discrete electrodes 17′ and an electrode controller. The pre-loaded carrier 10′ comprises an electrically insulating sheet 11′ having a front hydrophobic surface 11 a′ and a back surface. This electrically insulating sheet 11′ is removably attachable to a surface 16″ of the electrode array 16′ of the digital microfluidic device 14′. When positioned on the electrode array 16′ of the digital microfluidic device 14′, said electrically insulating sheet 11′ covers said discrete electrodes 17′ and provides electrical insulation to the discrete electrodes 17′ from each other and from liquid droplets 20′,22′,33′ present on the front hydrophobic surface 11 a′. The electrically insulating sheet 11′ according to a first embodiment of the present invention has one or more reagent depots 12′ located in one or more pre-selected positions 13′ on its front hydrophobic surface 11 a′. In operation, the electrode controller of the digital microfluidic device 14′ is capable of selectively actuating and de-actuating said discrete electrodes 17′ for translating liquid droplets 20′,22′,33′ over the front hydrophobic surface 11 a′ of the electrically insulating sheet 11′ and said one or more pre-selected positions 13′ on the front working surface 11 a′ of said electrically insulating sheet 11′ are positioned to be accessible to droplets 20′,22′,33′ actuated over the front hydrophobic surface 11 a′ of the electrically insulating sheet 11′. 

What is claimed is:
 1. A method of sample preparation comprising: providing an electrically insulating sheet comprising a hydrophobic surface, said hydrophobic surface having thereon a dried sample at a first location; contacting said electrically insulating sheet with a surface of a digital microfluidic device, wherein said first location is dropwise addressable under actuation of said digital microfluidic device; providing an extraction solvent at a second location, wherein said second location is dropwise addressable under actuation of said digital microfluidic device; and actuating said digital microfluidic device to transport a droplet of said extraction solvent to said first location for extracting an analyte in said dried sample; wherein the dried sample is selected from the group consisting of: dried whole blood, dried serum, dried plasma, dried urine, dried sputum, and dried cerebral spinal fluid.
 2. The method of claim 1, wherein an exchangeable carrier comprises said electrically insulating sheet.
 3. The method of claim 2, wherein said exchangeable carrier further comprises dried reagents disposed at one or more pre-selected positions on said surface of said electrically insulating sheet.
 4. The method of claim 3, wherein said one or more pre-selected positions on said hydrophobic surface are accessible to droplets actuated over said hydrophobic surface.
 5. The method of claim 1, further comprising incubating said droplet of said extraction solvent in said first location thereby extracting said analyte from said dried sample into said droplet of said extraction solvent.
 6. The method of claim 5, further comprising actuating said digital microfluidic device to transport said droplet of said extraction solvent comprising said analyte to a second array element.
 7. The method of claim 6, further comprising drying said droplet of said extraction solvent comprising said analyte, thereby providing a dried analyte spot at said second array element; electrically actuating said digital microfluidic device to provide a derivatization reagent droplet to said second array element, thereby contacting said dried analyte spot; and incubating said derivatization reagent droplet to solubilize and derivatize said dried analyte spot.
 8. The method of claim 7, further comprising evaporating said derivatization reagent droplet, thereby providing a dried derivatized analyte spot.
 9. The method according to claim 1, wherein said extraction solvent comprises a polar solvent.
 10. The method according to claim 1, wherein said analyte is selected from the group consisting of an amino acid, fatty acid, acylcarnitine, and organic acid.
 11. The method according to claim 1, wherein said analyte is a marker of a metabolic disorder.
 12. The method of claim 10, wherein said electrically insulating sheet is a non-porous solid phase.
 13. The method of claim 1, wherein said contacting said electrically insulating sheet with said surface of said digital microfluidic device further comprises contacting a channel-facing surface of a top plate or a bottom plate of a two-plate digital microfluidic device. 